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permeabilization blocking solution  (Biotium)


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    Structured Review

    Biotium permeabilization blocking solution
    Permeabilization Blocking Solution, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilization+blocking+solution/Permeabilization+and+Blocking+Buffer/pmc12399585-113-5-56
    Average 93 stars, based on 14 article reviews
    permeabilization blocking solution - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Whole Genome Amplification:

    Article Title: Whole Mount Imaging Methodology to Visualize and Quantify Peripheral Lens Structure, Cell Morphology, and Organization
    Article Snippet: .. 1.3.1 Place fixed lenses in a 0.5 mL solution of rhodamine-phalloidin (1:300, Thermo Fisher Scientific), WGA-640 (1:250, Biotium), and Hoechst 33342 (1:500, Biotium) in permeabilization/blocking solution (3% BSA, 3% goat serum and 0.3% Triton) within a microcentrifuge tube. ..

    Article Title: TPM3.1 association with actin stress fibers is required for lens epithelial to mesenchymal transition
    Article Snippet: .. Lenses were then washed three times in PBS and placed in permeabilization/blocking solution containing Alexa 488-conjugated anti-mouse secondary antibody (1:200), CF640 dye-conjugated to wheat germ agglutinin (WGA) (Biotium, Fremont, CA) (1:100), rhodamine-phalloidin (1:20), and Hoechst 33342 (1:500) at room temperature for 2 hours. ..

    Article Title: Tropomyosin 3.1 Association With Actin Stress Fibers is Required for Lens Epithelial to Mesenchymal Transition
    Article Snippet: .. Lenses were then washed three times in PBS and placed in permeabilization/blocking solution containing Alexa 488-conjugated anti-mouse secondary antibody (1:200), CF640 dye-conjugated to wheat germ agglutinin (WGA; 1:100; Biotium, Fremont, CA, USA), rhodamine-phalloidin (1:20), and Hoechst 33342 (1:500) at room temperature for 2 hours. ..

    Staining:

    Article Title: Enhanced Superficial Zone Chondrocyte Expansion and Redifferentiation by Culture on Chondrocyte-Derived Decellularized Matrices
    Article Snippet: After 24 hours, cells were fixed by incubating cells in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) at room temperature for 15 minutes, and were then washed 3 times with PBS−/−. .. Cells were then permeabilized using permeabilization/blocking solution (3% Goat serum, 3% BSA, and 0.3% Triton) for 30 min. To visualize globular (G-) and filamentous (F-) actin, P2 SZC on PS and CD-ECM coverslips were stained with vitamin D binding protein conjugated to Alexa 488 (G-actin; VitDBP-488; 1:100; RayBiotech, Peachtree Corners, GA, USA) and rhodamine-phalloidin (F-Actin; 1:50; Biotium). .. Cells were counterstained with Hoechst 33342 (1/500; Biotium).

    Binding Assay:

    Article Title: Enhanced Superficial Zone Chondrocyte Expansion and Redifferentiation by Culture on Chondrocyte-Derived Decellularized Matrices
    Article Snippet: After 24 hours, cells were fixed by incubating cells in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) at room temperature for 15 minutes, and were then washed 3 times with PBS−/−. .. Cells were then permeabilized using permeabilization/blocking solution (3% Goat serum, 3% BSA, and 0.3% Triton) for 30 min. To visualize globular (G-) and filamentous (F-) actin, P2 SZC on PS and CD-ECM coverslips were stained with vitamin D binding protein conjugated to Alexa 488 (G-actin; VitDBP-488; 1:100; RayBiotech, Peachtree Corners, GA, USA) and rhodamine-phalloidin (F-Actin; 1:50; Biotium). .. Cells were counterstained with Hoechst 33342 (1/500; Biotium).



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